Secretion to the growth medium of an alpha-amylase by Escherichia coli clones carrying a Bacillus laterosporus gene.

Manonmani, H. K. and Kunhi, A. A. M. (1999) Secretion to the growth medium of an alpha-amylase by Escherichia coli clones carrying a Bacillus laterosporus gene. World Journal of Microbiology & Biotechnology, 15 (4). 475-480, 19 ref..

PDF World_Journal_of_Microbiology_&_Biotechnology_15_475-480,_1999.pdf Restricted to Registered users only Download (120kB)

Abstract

The alpha-amylase gene from Bacillus laterosporus P3 was cloned and expressed in Escherichia coli HB101 and DH5alpha. Up to 92% of the cloned gene product was secreted into the medium by recombinant E. coli. The recombinant crude enzyme showed improved functionality in terms of activity at a wider pH range and at higher temp. compared with the crude enzyme from the donor strain. Improved functionality of the cloned enzyme was due to the absence of a contaminating proteinase which was co-produced in the donor strain. Sub-cloning of the alpha-amylase gene using the promoter-probe vector pKT240 in E. coli DH5alpha indicated the presence of a promoter of B. laterosporus P3 in the cloned fragment.

Item Type:	Article
Uncontrolled Keywords:	AMYLASES-; BACILLUS-; ESCHERICHIA-; GENE-CLONING; GENETICS-; MICROORGANISMS-; alpha-AMYLASES; BACILLUS-LATEROSPORUS; ESCHERICHIA-COLI; RECOMBINANT-MICROORGANISMS
Subjects:	600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes
Divisions:	Food Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	30 Jun 2009 09:45
Last Modified:	14 Dec 2016 12:23
URI:	http://ir.cftri.res.in/id/eprint/7726

Actions (login required)

View Item