Production of Bacillus thuringiensis var. kurstaki by three different methods and its relative toxicity to Bombyx mori.

Mummigatti, S. G. and Raghunathan, A. N. (1988) Production of Bacillus thuringiensis var. kurstaki by three different methods and its relative toxicity to Bombyx mori. Journal of Invertebrate Pathology, 51 (2). 115-118; 5 ref..

PDF Journal of Invertebrate Pathology, Volume 51, Issue 2, March 1988, Pages 115-118.pdf - Published Version Restricted to Registered users only Download (345kB)

Abstract

The relative toxicity of the entomopathogenic bacterium Bacillus thuringiensis subsp. kurstaki produced in submerged culture, surface culture using modified tryptone medium or in vivo using 3rd-instar larvae of Bombyx mori was evaluated against 3rd-instar larvae of B. mori. The submerged culture method was the most effective, producing 272.5 X 103 toxicity units, followed by the surface culture and in vivo methods, which produced 121.6 X 103/litre medium and 9.1 X 103 toxicity units/kg medium, respectively. Higher biomass and unfavourable conditions resulted in the in vivo method having the lowest production (5 X 109/g) of spore crystals. The surface culture method had the highest production (62 X 109/g). Products from submerged and surface cultures were 29.9 and 13.4 times as potent as those from the in vivo method.

Item Type:	Article
Uncontrolled Keywords:	Microbial-pesticides; Pathogens-; Beneficial-insects; Natural-enemies; Entomopathogenic-bacteria; culture-techniques; insecticides-; pathogenicity-; hosts-; Toxins-; Toxicity-; Entomopathogens-; techniques-; agricultural-entomology; biological-control-agents
Subjects:	600 Technology > 01 Medical sciences > 17 Toxicology
Divisions:	Food Protectants and Infestation Control
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	30 Mar 2016 10:10
Last Modified:	30 Mar 2016 10:10
URI:	http://ir.cftri.res.in/id/eprint/8211

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