Effect of different cultural conditions for phytase production by Aspergillus niger CFR 335 in submerged and solid-state fermentations

Gunashree, B. S. and Venkateswaran, G. (2008) Effect of different cultural conditions for phytase production by Aspergillus niger CFR 335 in submerged and solid-state fermentations. Journal of Industrial Microbiology Biotechnology, 35. pp. 1587-1596.

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Abstract

The present article deals with the studies on the eVect of media ingredients, such as carbon, nitrogen, inorganic phosphates, surfactants, and metal salts, on phytase enzyme production by Aspergillus niger CFR 335 in submerged (SmF) and solid-state fermentations (SSF). The results obtained showed a 1.5-fold higher enzyme yield in the presence of sucrose in both SmF and SSF, while peptone was found to be a favorable nitrogen source for SmF. Sodium dihydrogen phosphate (NaH2PO4) favored 34% higher enzyme yield than the control, which was followed by 19% higher activity in potassium dihydrogen phosphate (KH2PO4) in SSF at 0.015% w/v. The addition of Tween- 20 in SmF showed a maximum yield of 12.6 U/mL while, SDS suppressed the growth of the fungus. None of the surfactants favored the enzyme yield in SSF. Calcium chloride (CaCl2) was extensively eYcient in stimulating more than 55% higher phytase production in SmF at 0.01% v/v. In SSF, none of the metal salts stimulated phytase production.

Item Type:	Article
Uncontrolled Keywords:	Extracellular phytase · Phytic acid · Monogastrics · Aspergillus niger CFR 335 · Media ingredients
Subjects:	600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes
Divisions:	Food Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	05 Jan 2009 10:46
Last Modified:	28 Dec 2011 10:07
URI:	http://ir.cftri.res.in/id/eprint/8875

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