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Cultivation of Dunaliella Bardawil Rich in Carotenoids and Studies on Nutritional and Biological Activities
Vanitha, A. (2007) Cultivation of Dunaliella Bardawil Rich in Carotenoids and Studies on Nutritional and Biological Activities. PhD thesis, University of Mysore.
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Abstract
Carotenoids are being intensively investigated regarding their potential to prevent chronic diseases and vitamin A deficiency. Dunaliella is a eukaryotic photosynthetic micro-algae belonging to Chlorophyceae, which can produce β-carotene in large quantities under stress conditions. The study presented in this thesis consists of development of a cultivation methodology for the production of D. bardawil in outdoor conditions, determination of its efficiency to accumulate carotenoids under different experimental conditions, evaluation of stability of carotenoids in biomass, safety analysis of biomass in rat models, evaluation of its biological activity, bioavailability and vitamin A conversion efficiency in rat experimental models. An efficient outdoor cultivation and harvesting methodology was developed for both in indoor and outdoor condition. D. bardawil produced upto 4% β-carotene in outdoor culture condition. Analysis of the biomass revealed 22% protein, 27% carbohydrate, and 8 % fat content on dry weight basis. D. bardawil cultures when exposed to high light intensity of 30-35 Klux showed accumulation of carotenoids. Among the carotenoids, β-carotene was predominant along with other carotenoids such as lutein, lycopene and α-carotene. The enhancement of β- carotene accumulation positively correlated with the up regulation of phytoene synthase, phytoene desaturase and lycopene cyclase mRNA transcripts when the cells were subjected to high light intensity. Feeding trials, for experimental rats, were carried out to assess the safety and efficacy of D. bardawil biomass as a source of β-carotene. The study revealed that D. bardawil biomass was safe in single (5g kg-1 body weight) as well as multiple doses (100mg & 1000mg kg-1 body weight for 90 days) in experimental rats. In vivo experiments demonstrated that D. bardawil β-carotene is as efficient as synthetic β-carotene in terms of retinol formation and its accumulation in serum and liver. D. bardawil β-carotene showed higher level of β-carotene accumulation in liver compared to synthetic β-carotene in case of repeated doses. The carotenoids of D. bardawil biomass were as efficient as synthetic β-carotene, against CCl4 induced hepatic and renal damage. This study demonstrated that D. bardawil could be mass cultivated to produce β-carotene rich biomass that could be safely used as a nutritional supplement.
| Item Type: | Thesis (PhD) |
|---|---|
| Uncontrolled Keywords: | Carotenoids Dunaliella micro-algae cultivation methodology biological activity |
| Subjects: | 600 Technology > 05 Chemical engineering > 01 Biotechnology and Bioengineering 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 01 Algae |
| Divisions: | Plant Cell Biotechnology |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 07 May 2009 06:23 |
| Last Modified: | 28 Dec 2011 10:08 |
| URI: | http://ir.cftri.res.in/id/eprint/8930 |
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