Serological evidence for the occurrence of races in Sclerospora graminicola and identification of a race-specific surface protein involved in host recognition

Vasantha, U. Kumar and Shishupala, S. and Shetty, H. S. (1993) Serological evidence for the occurrence of races in Sclerospora graminicola and identification of a race-specific surface protein involved in host recognition. Canadian Journal of Botany, 71. pp. 1467-1471.

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Abstract

A protein specific to a virulent pathotype of the downy mildew pathogen Sclerospora grarninicola was identified on the Western transfers using the cross-adsorbed antibody. This protein was approximately 90 kDa and without subunits. Serological agglutination studies localized the protein on the cell wall surfaces. ~ n z ~ mlineke d immunosorbent assay and dot blotting using the specific antibodies identified distinct races of the pathogen. Immunofluorescence studies and enzyme linked immunosorbent assay using suspension cultures of host cells showed that the protein bound to the cells of susceptible cultivar but not to those of resistant cultivar. These results suggested that this protein may be involved in host recognition.

Item Type:	Article
Uncontrolled Keywords:	Sclerospora graminicola, Penniseturn glaucum, host-pathogen recognition
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
Divisions:	Food Protectants and Infestation Control
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	24 Feb 2010 07:04
Last Modified:	11 Oct 2018 07:01
URI:	http://ir.cftri.res.in/id/eprint/9310

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