Studies on the production of nigerloxin using agro-industrial residues by solid-state fermentation

Dasagrandhi, Chakradhar and Saleem, Javeed and Avinash, P. Sattur (2009) Studies on the production of nigerloxin using agro-industrial residues by solid-state fermentation. Journal of Industrial Microbiology and Biotechnology, 36. pp. 1179-1187.

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Abstract

Nigerloxin, a new and potent lipoxygenase inhibitor, was discovered in our laboratory through solidstate fermentation of wheat bran by Aspergillus niger V. Teigh (MTCC-5166). The aim of this study is to investigate the possibility of using different agro-industrial residues as nutritional supplements along with wheat bran to enhance the production of nigerloxin. Nigerloxin produced by SSF was quantified spectrophotometrically at 292 nm. The results indicate that the inhibitor production was influenced by the type of solid substrate supplemented, moisture content, pH and size of the inoculum. Individually optimized supplements were tested in different combinations to determine their effects on nigerloxin production. A twofold increase in the production of nigerloxin (4.9 ± 0.3 mg gds-1) was achieved by supplementing wheat bran with 10% w/w sweet lemon peel and 5% v/w methanol at optimized process parameters, that is, an initial moisture content of 65% v/w and incubation period of 6 days with an initial inoculum size of 2 ml (8 9 105 spores gds-1). Nigerloxin production was stable between pH of 4 and 5.

Item Type:	Article
Uncontrolled Keywords:	Agro-industrial residues Solid-state fermentation Aspergillus niger V. Teigh (MTCC-5166) Nigerloxin Lipoxygenase inhibitor
Subjects:	600 Technology > 05 Chemical engineering > 04 Fermentation Technology
Divisions:	Fermentation Technology and Bioengineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	26 Feb 2010 10:49
Last Modified:	28 Dec 2011 10:13
URI:	http://ir.cftri.res.in/id/eprint/9363

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