Evaluation of Sublethal Effects of Dichlorvos upon Caenorhabditis elegans Based on a Set of End Points of Toxicity

Kisan, B. Jadhav and Rajini, P. S. (2009) Evaluation of Sublethal Effects of Dichlorvos upon Caenorhabditis elegans Based on a Set of End Points of Toxicity. Journal of Biochemical and Molecular Toxicology, 23 (1). pp. 9-17.

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Abstract

The primary objective of this study was to examine a possible correlation among the three endpoints of toxicity, namely, stress gene expression (hsp16), feeding, and acetylcholinesterase (AChE) activity in transgenic C. elegans (hsp16-lacZ) exposed to sublethal concentrations of dichlorvos, an organophosphorus insecticide.Worms exposed to dichlorvos (at 5, 40, and 80 μM) exhibited a concentration-dependent inhibition in feeding with total cessation in feeding occurring beyond 4 h of exposure. Concomitantly, marked and dose-dependent inhibition (69%–90%) of AChE was also evident after 4 h of exposure. Induction of heat shock protein (Hsp) was evident after 4 h of exposure (as seen from quantitative analysis), although maximum expression of Hsp was evident only after 24 h of exposure (as evident from qualitative analysis). Interestingly, the Hsp induction was restricted only to the pharyngeal region. Significant correlation was discernible between the three evaluated end points suggesting their possible interrelated role in the physiological dysfunctions evoked by sublethal concentrations of dichlorvos.

Item Type:	Article
Uncontrolled Keywords:	Transgenic Caenorhabditis elegans; hsp16; Dichlorvos; AChE Inhibition, Feeding; Sublethal Concentrations
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 26 Pesticide Chemistry
Divisions:	Food Protectants and Infestation Control
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	26 Feb 2010 10:57
Last Modified:	01 Jun 2012 11:13
URI:	http://ir.cftri.res.in/id/eprint/9365

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