Micropropagation of Bixa orellana using phytohormones and triacontanol

Parimalan, R. and Giridhar, P. and Gururaj, H. B. and Ravishankar, G. A. (2009) Micropropagation of Bixa orellana using phytohormones and triacontanol. Biologia Plantarum, 53 (2). pp. 347-350.

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Abstract

An efficient micropropagation protocol for annatto (Bixa orellana L.) was achieved using nodal shoot tip explants. Shoot buds were obtained on the Murashige and Skoog (MS) medium supplemented with various concentrations and combinations of indole-3-acetic acid (IAA), N6-benzyladenine (BA) and triacontanol (TRIA). Maximum of 213 shoot buds along with 18 primary shoots were produced on MS medium containing 0.05 μM IAA, 8.87 μM BA, and 11.2 μM TRIA. The primary shoots elongated best on MS medium containing 6.66 μM BA and 2.45 μM indole-3-butyric acid (IBA). The regenerated shoots rooted best on MS medium supplemented with 4.9 μM IBA. The in vitro rooted plantlets were hardened and establishment rate under field conditions was 70 to 80 %.

Item Type:	Article
Uncontrolled Keywords:	annatto, callus, ex vitro transfer, multiple shoots, organogenesis
Subjects:	500 Natural Sciences and Mathematics > 10 Plants > 06 Trees And Shrubs 500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
Divisions:	Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	12 Nov 2010 05:38
Last Modified:	09 Apr 2018 11:19
URI:	http://ir.cftri.res.in/id/eprint/9770

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