Identification of Carotenoids from Green Alga Haematococcus pluvialis by HPLC and LC-MS (APCI) and Their Antioxidant Properties

Ranga, Rao and Sarada, A. R. and Baskaran, V. and Ravishankar, G. A. (2009) Identification of Carotenoids from Green Alga Haematococcus pluvialis by HPLC and LC-MS (APCI) and Their Antioxidant Properties. Journal of Microbiology and Biotechnology, 19 (11). pp. 1333-1341.

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Abstract

Haematococcus pluvialis, a green alga, accumulates astaxanthin (3,3'-dihydroxy-β,β'-carotene-4,4'-dione) upto 2-3% on a dry weight basis. In the present study, identification of carotenoids from Haematococcus cyst cell extract by HPLC and LC-MS (APCI) and their antioxidant properties were evaluated in in vitro model systems. The extract exhibited 89% and 78% antioxidant activities in the β-carotene linoleate model and the hydroxyl radical scavenging model, at 9 ppm of total carotenoid, respectively. The extract also showed 80%, 85%, and 79% antioxidant activities against lipid peroxidation in the kidney, brain, and liver of rats. Low-density lipoprotein oxidation induced by Cu2+ ions was also protected (45%, 64%, and 75%) by the extract in a dose-dependent manner with different carotenoid levels. Thiobarbituric acid reactive substances concentration in the blood, liver, and kidney of rats were also significantly (p<0.005) decreased in H. pluvialis-treated rats. The potent antioxidant activity is attributable to various carotenoids present in the extract.

Item Type:	Article
Uncontrolled Keywords:	H. pluvialis, carotenoids, antioxidant properties, HPLC, LC-MS (APCI)
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 06 Chromatography 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 01 Algae 600 Technology > 08 Food technology > 32 Antioxidants
Divisions:	Plant Cell Biotechnology Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	12 Nov 2010 09:41
Last Modified:	28 Dec 2011 10:19
URI:	http://ir.cftri.res.in/id/eprint/9775

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