In vitro Antioxidant and Antibacterial Properties of Hydrolysed Proteins of Delimed Tannery Fleshings: Comparison of Acid Hydrolysis and Fermentation Methods

Bijinu, B. and Binod, Prasad and Amit Kumar, Rai and Suresh, P. V. and Mahendrakar, N. S. and Bhaskar, N. (2011) In vitro Antioxidant and Antibacterial Properties of Hydrolysed Proteins of Delimed Tannery Fleshings: Comparison of Acid Hydrolysis and Fermentation Methods. Biodegradation, 22 (2). pp. 287-295.

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Abstract

Proteins in delimed tannery fleshings were fermentatively hydrolysed using Enterococcus faecium NCIM5335 and also hydrolysed using mild organic acids (formic acid and propionic acid). The liquor portion containing hydrolysed proteins was spray dried, in both the cases, to obtain a powder. The spray dried powder was evaluated for in vitro antioxidant activities with respect to scavenging different free radicals and antibacterial properties against nine different pathogens. Fermentation and acid hydrolysates scavenged 83 and 75.3% of 2,2-azino-bis-3- ethyl-benzthiazoline-6-sulphonic acid (ABTS) radicals, respectively, at a protein concentration of 0.25 mg. Further, fermentation hydrolysate showed higher 2,2- diphenyl-1-picrylhydrazyl radical scavenging activity of 59% as compared to 56% scavenging by acid hydrolysate at a protein concentration of 5 mg. Acid hydrolysate exhibited lesser (82.3%) peroxy radical scavenging compared to hydrolysate from fermentation (88.2%) at a protein concentration of 10 mg. However, acid hydrolysate exhibited higher (89.2%) superoxide anion scavenging while its fermentation counterpart showed lower activity (85.4%) at 2.5 mg hydrolysate protein. Well as superoxide anion scavenging properties. All the in vitro antioxidant properties exhibited dose dependency. Fermentation hydrolysate exhibited maximum antagonistic activity against Salmonella typhi FB231, from among host of pathogens evaluated. Both the hydrolysates have potential to be ingredients in animal feeds and can help reduce oxidative stress in the animals.

Item Type:	Article
Uncontrolled Keywords:	Tannery fleshings Fermentation Acid hydrolysis Antioxidative Antibacterial
Subjects:	600 Technology > 05 Chemical engineering > 04 Fermentation Technology
Divisions:	Meat Fish and Poultry Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	04 Feb 2011 09:33
Last Modified:	04 Dec 2017 09:06
URI:	http://ir.cftri.res.in/id/eprint/9881

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