Ashwani, Sharma and Vinod, Kumar and Giridhar, Parvatam and Ravishankar, G. A. (2008) Induction of in vitro flowering in Capsicum frutescens under the influence of silver nitrate and cobalt chloride and pollen transformation. Electronic Journal of Biotechnology, 11 (2). pp. 1-6.
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Abstract
The influence of silver nitrate (AgNO3) and cobalt
chloride (CoCl2) on shoot multiplication and in vitro
flowering in Capsicum frutescens Mill. was investigated.
Exogenous administration of AgNO3 and CoCl2 at a
concentration of 30 μM resulted in the maximum tissue
response in terms of shoot length and number of shoots
after 45 days culturing on MS medium. Both silver
nitrate (40 μM) and cobalt chloride (30 μM) influenced
in vitro flowering after 25 and 45 days respectively. This
is the first report on in vitro flowering in C. frutescens.
The study also demonstrated successful transformation
of pollen obtained from the in vitro flowers. Since
capsicum is highly recalcitrant to in vitro plant
regeneration, the results of the study may be highly
useful in transformation of capsicum using germ free in
vitro flowers.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Capsicum frutescens, cobalt chloride, in vitro flowering, pollen transformation, regeneration, silver nitrate. |
| Subjects: | 600 Technology > 08 Food technology > 30 Spices/Condiments 500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture |
| Divisions: | Plant Cell Biotechnology |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 07 Jun 2011 06:40 |
| Last Modified: | 28 Dec 2011 10:23 |
| URI: | http://ir.cftri.res.in/id/eprint/10078 |
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