In Vitro Shoot Multiplication Through Shoot Tip Cultures of Decalepis Hamiltonii Wight & Arn., A Threatened Plant Endemic to Southern India

Giridhar, P. and Gururaj, H. B. and Ravishankar, G. A. (2005) In Vitro Shoot Multiplication Through Shoot Tip Cultures of Decalepis Hamiltonii Wight & Arn., A Threatened Plant Endemic to Southern India. In Vitro Cellular and Developmental Biology - Plant, 41. pp. 77-80.

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Abstract

An efficient and rapid micropropagation system was developed for a food and medicinally important endangered shrub, Decalepis hamiltonii (‘swallow root’), through shoot multiplication. The influence of 2.5–7.5 mM isopentenyladenine (2iP), 4.4–17.7mM 6-benzyladenine, 2.3–4.7 mM kinetin, 2.8–6.8mM thidiazuron, and 2.3–11.4mM zeatin alone and in combination with 0.3–0.9 mM indole-3-acetic acid (IAA) on in vitro multiple shoot production was studied. The maximum number of multiple shoots (6.5 ^ 0.4) was induced from shoot tips cultured on agar-based Murashige and Skoog (MS) medium containing 4.9 mM 2iP. But, both zeatin (9.1 mM) and kinetin (4.7mM) in combination with IAA (0.6 mM) were able to produce a maximum of 5.0 ^ 0.4 and 5.l ^ 0.4 multiple shoots, respectively. Further elongation of shoots and adventitious shoot formation was obtained on medium containing 2.5 mM 2iP and 0.3mM gibberellic acid. Elongated shoots were separated and rooted on MS medium supplemented with 9.8 mM indole-3-butyric acid (IBA) and various phenolic compounds within 5–6 wk. Phloroglucinol and salicylic acid interaction with IBA stimulated in vitro rooting of shoots. Successful field transfer was achieved in rooted plantlets

Item Type:	Article
Uncontrolled Keywords:	kinetin; rooting; shoot multiplication; shoot tips; zeatin.
Subjects:	500 Natural Sciences and Mathematics > 10 Plants > 06 Trees And Shrubs 500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
Divisions:	Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	29 Jul 2011 11:20
Last Modified:	28 Dec 2011 10:26
URI:	http://ir.cftri.res.in/id/eprint/10322

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