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Endogenous profiles of indoleamines: serotonin and melatonin in different tissues of Coffea canephora P ex Fr. as analyzed by HPLC and LC-MS-ESI. fermentation: parameter optimization and application for N-acetyl chitooligosaccharides preparation from chitin.
Ramakrishna, Akula and Giridhar, P. and Udaya Sankar, K. and Ravishankar, G. A. (2012) Endogenous profiles of indoleamines: serotonin and melatonin in different tissues of Coffea canephora P ex Fr. as analyzed by HPLC and LC-MS-ESI. fermentation: parameter optimization and application for N-acetyl chitooligosaccharides preparation from chitin. Acta Physiologiae Plantarum, 34. 393–-396.
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Abstract
Endogenous indoleamine profiles in various ex vitro and in vitro tissues of commercially important Coffea canephora were analyzed by using a high performance liquid chromatography and further confirmed with electrospray ionization mass spectrometry. High content of serotonin (SER) (98.54 ± 5 lg/g) and melatonin (MEL) (115.25 ± 6 lg/g) were found in freshly harvested seeds of C. canephora followed by zygotic embryo (65.25 ± 4 and 96.54 ± 5 lg/g freshweight) and endosperm(34.08 ± 2 and 51.08 ± 4 lg/g fresh weight) of ripened fruits. Similarly endogenous pools of SER and MEL were moderate in in vitro tissues of C. canephora, i.e. callus (25.85 ± 2 and 75.74 ± 4), somatic embryos (31.88 ± 2 and 19.30 ±2 lg/g fresh weight) and in vitro regenerated plant stalk (15.78 ± 1 and 38.25 ± 3 lg/g fresh weight), respectively. In view of significant levels of both SER and MEL in various tissues and beans of Coffea, further investigations on their physiological role needs to be investigated.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Indoleamines Somatic embryos Tissue culture Zygotic embryos |
| Subjects: | 500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture 600 Technology > 07 Beverage Technology > 04 Coffee |
| Divisions: | Plant Cell Biotechnology |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 05 Aug 2011 11:40 |
| Last Modified: | 14 Feb 2012 09:47 |
| URI: | http://ir.cftri.res.in/id/eprint/10420 |
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