Microplate-based kinetic method for assay of mitochondrial NADH– and succinate–cytochrome c reductase activities.

Apurva Kumar, R. J. and Raju, N. and Rajini, P. S. (2011) Microplate-based kinetic method for assay of mitochondrial NADH– and succinate–cytochrome c reductase activities. Analytical Biochemistry , 415. pp. 209-211.

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Abstract

This article describes a microplate-based kinetic assay for mitochondrial NADH– and succinate–cytochrome c reductase activities in rat brain mitochondria. The assay reported here is based on the conventional spectrophotometric method and involves substrate-driven reduction of exogenous cytochrome c. Conditions regarding linearity with respect to time and protein concentration have been standardized. Furthermore, the methods were tested for inhibition of respective activities by specific inhibitors. The microplate format described here can be employed for rapid and simultaneous measurements of mitochondrial NADH– and succinate–cytochrome c reductase activities in a large number of samples.

Item Type:	Article
Uncontrolled Keywords:	NADH–cytochrome c reductase, succinate–cytochrome c reductase, microplate-based kinetic method
Subjects:	600 Technology > 01 Medical sciences > 17 Toxicology
Divisions:	Food Protectants and Infestation Control
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	10 Feb 2012 03:56
Last Modified:	28 Jun 2016 08:23
URI:	http://ir.cftri.res.in/id/eprint/10591

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