Direct shoot bud induction and plant regeneration in Capsicum frutescens Mill.: influence of polyamines and polarity.

Direct shoot bud induction and plant
regeneration was achieved in Capsicum frutescens var.
KTOC. Aseptically grown seedling explants devoid of
roots, apical meristem and cotyledons were inoculated
in an inverted position in medium comprising of Murashige
and Skoog (Physiol Plant 15:472–497, 1962)
basal medium supplemented with 2-(N-morpholine)
ethanesulphonic acid buffer along with 2.28 lM indole-
3-acetic acid, 10 lM silver nitrate and either of 13.31–
89.77 lM benzyl adenine (BA), 9.29–23.23 lM kinetin,
0.91–9.12 lM zeatin, 2.46–9.84 lM 2-isopentenyl adenine.
Profuse shoot bud induction was observed only in
explants grown on a media supplemented with BA
(26.63 lM) as a cytokinin source and 19.4 ± 4.2 shoot
buds per explant was obtained in inverted mode under
continuous light. Incorporation of polyamine inhibitors
in the culture medium completely inhibited shoothoot
bud induction. Incorporation of exogenous polyamines
improved the induction of shoot buds under 24 h
photoperiod. These buds were elongated in MS medium
containing 2.8 lM gibberellic acid. Transfer of
these shoots to hormone-free MS medium resulted in
rooting and rooted plants were transferred to fields.
This protocol can be efficiently used for mass propagation
and presumably also for regeneration of genetically
transformed C. frutescens.