Osmolarity, conductivity, buffer capacity, and solubility of oxygen of plant cell culture media.

Osmolarity, conductivity, buffer capacity, and solubility of oxygen of twelve commonly used plant tissue
and cell culture media were investigated. Osmolarity and conductivity of different nutrient media showed
con-siderable difference. Buffer capacity of culture medium measured from acid-base titration value indicated
that plant cell culture media had moderate buffer strength. It varied from 82 mole/L of White's medium to
1564 mole/L of Eriksson's medium pH 5.5. Solubility of oxygen in various celt culture media ranged from 7.01
-7.79 mg/L. The present data would be useful in bioprocessing and in the selection of nutrient medium for in
vi-tro culture of plant tissue and cells.