Quantum dots as nanoplug-in's for efficient NADH resonance energy routing.

Akshath, U. S. and Vinayaka, A. C. and Thakur, M. S. (2012) Quantum dots as nanoplug-in's for efficient NADH resonance energy routing. Biosensors and Bioelectronics, 38. pp. 411-415.

PDF Biosensors and Bioelectronics, Volume 38, Issue 1, October–December 2012, Pages 411-415.pdf - Published Version Restricted to Registered users only Download (349kB)

Abstract

The routing of fluorescent signals from NADH to quantum dots (QDs) has been a subject of extensive research for FRET based applications. In the present study, the spectral cross talk of NAD+/NADH with QDs was used to monitor the reaction of NAD+-dependent dehydrogenase enzyme. CdTe QD may undergo dipolar interaction with NADH as a result of broad spectral absorption due to multiple excitonic states resulting from quantum confinement effects. Thus, non-radiative energy transfer can take place from NADH to CdTe QD enhancing QDs fluorescence. Energyrouting assay of NADH-QD was applied for detection of formaldehyde as a model analyte in the range 1000–0.01 ng/mL by the proposed technique. We observed proportionate quenching of CdTe QD fluorescence by NAD+ and enhancement in the presence of NADH formed by various concentrations of enzyme (0.028–0.4 U). Hence, it was possible to detect formaldehyde in the range 1000−0.01 ng/mL with a limit of detection (LOD) at 0.01 ng/mL and regression coefficient R2=0.9982. Therefore, a unique optical sensor was developed for the detection of the formaldehyde in sensitive level based on the above mechanism. This method can be used to follow the activity of NAD+-dependent enzymes and detection of dehydrogenases in general.

Item Type:	Article
Uncontrolled Keywords:	Quantum dots, NADþ/NADH, FRET Formaldehyde dehydrogenase
Subjects:	600 Technology > 05 Chemical engineering > 01 Biotechnology and Bioengineering
Divisions:	Fermentation Technology and Bioengineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	27 Aug 2012 06:18
Last Modified:	27 Aug 2012 06:18
URI:	http://ir.cftri.res.in/id/eprint/10958

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