Priyanka, B. S. and Rastogi, N. K. and Raghavarao, K.S.M.S. and Thakur, M. S. (2012) Downstream processing of luciferase from fireflies (Photinus pyralis) using aqueous two-phase extraction. Process Biochemistry, 47 . pp. 1358-1363. ISSN 1359-5113
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Abstract
The firefly luciferase has been extensively used for sensitive detection of bacteria, gene expression and
environmental toxins (biosensors). The aim of the present study was to design a simple and more efficient
method for the purification and concentration of luciferase using aqueous two-phase extraction (ATPE).
Downstream processing of luciferase from North American Firefly Photinus pyralis was carried out, for
the first time, using polymer/salt aqueous two phase system (ATPS) at 4 ◦C. The enzyme was observed to
preferentially partition to the polyethylene glycol (PEG) rich top phase. The best results of purification
(13.69 fold) and enzyme activity recovery (118.34%) were observed in the system containing 4.0% (w/w)
PEG (1500) and 20.5% (w/w) (NH4)2SO4 with a phase volume ratio of 0.21.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Downstream processing Luciferase, Bioseparation, Aqueous two-phase extraction Purification |
| Subjects: | 600 Technology > 08 Food technology > 07 Food Engineering |
| Divisions: | Fermentation Technology and Bioengineering Food Engineering |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 28 Aug 2012 06:44 |
| Last Modified: | 29 Dec 2016 13:11 |
| URI: | http://ir.cftri.res.in/id/eprint/10981 |
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