Detection of mobile genetic elements in pediocin PA-1 like producing lactic acid bacteria.

Manjulata Devi, S. and Prakash, M. Halami (2013) Detection of mobile genetic elements in pediocin PA-1 like producing lactic acid bacteria. Journal of Basic Microbiology , 53 (7). pp. 555-561.

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Abstract

To evaluate the presence of mobile genetic elements in intergeneric and interspecific pediocin producing lactic acid bacteria (LAB) the flanking regions of the pediocin PA-1/AcH (pediocin PA-1) operon was characterized. In Enterococcus faecium Acr4 and Lactobacillus plantarum Acr2 a variation in the amplicon size in the downstream region of the operon was identified, suggesting a deletion in this region. Beyond that, in pediocin PA-1 encoding plasmids mobile genetic elements such as ISLpl1 and mobilization regions were detected by Southern hybridization analysis. Phylogenetic analyses of the E. faecium Acr4 ISLpl1 gene sequence suggested the gene transfer from lactobacilli in the environment. The tyrosine recombinase detected in pediocin plasmids of P. acidilactici H and K7 indicate a possible transfer of the entire operon among LAB. Since these elements are known to be associated with transfer of genes linked to the bacteriocin production, antibiotic resistance and sugar utilization, we suggest similar mechanism for natural spread of pediocin PA-1 operon among different bacterial species.

Item Type:	Article
Uncontrolled Keywords:	Lactic acid bacteria Pediocin PA-1 Mobile genetic elements ISlpl1 Plasmids
Subjects:	600 Technology > 08 Food technology > 09 Food Microbiology
Divisions:	Food Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	16 Nov 2012 08:17
Last Modified:	22 Dec 2016 12:17
URI:	http://ir.cftri.res.in/id/eprint/11016

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