Integrated Approach for the Extraction and Purification of IgY from Chicken Egg Yolk.

The production of Immunoglobulin Y (IgY) from an
immunized chicken egg yolk serves numerous applications in
various fields of immunology. The present work, for the first
time, demonstrates the feasibility of aqueous two-phase extraction
and three phase partitioning for the separation of IgY from the
chicken egg yolk. The protocol deals with partitioning of IgY
using polyethylene glycol 1500/potassium phosphate aqueous twophase
system and its comparison with three phase partitioning,
both followed by the precipitation. Sodium dodecyl sulphate
polyacrylamide gel electrophoresis was carried out to confirm the
presence of IgY at the different steps of purification process. The
IgY was preferentially partitioned to the PEG-rich top phase,
especially at lower phase-volume ratio. The effect of process
parameters such as tie line length and volume ratio was studied.
The tie line length of 5.55%and volume ratio of 0.49 gave the best
results. The yield of IgY obtained from aqueous two-phase system
was 9.0mg/mL of egg yolk while that of three phase partitioning
it was 6.0mg/mL.