Effect of dehydroabietylamine in angiogenesis and GSK3-b inhibition during wound healing activity in rats.

The present research evaluates the wound healing
capacity of dehydroabietylamine, a diterpenoid isolated
from methanolic leaves extract of Carthamus tinctorius L.
(Asteraceae). The wound healing activity was screened by
excision, incision, and dead space wound models on albino
rats and for the angiogenic property using chick chorioallantoic
membrane (CAM) assay. Significant increase in
wound contraction rate, skin breaking strength, granuloma
strength, and dry granuloma weights was observed as
compared to control. The pro-healing action may be due to
increasing in collagen deposition as well better alignment
and maturation. In CAM assay, the dehydroabietylamine
showed increased angiogenic zone formation in the developing
embryos compared to normal embryos and evidenced
pro-angiogenic activity of the active constituent. Further, in
silico comparative docking of dehydroabietylamine, standard
drug nitrofurazone and positive control CHIR_98014 to
ATP-binding active pocket of glycogen synthase kinase3-b
(GSK3-b) protein that consisted of Tyr216 as a catalytic
residue, with residues Asn64, Gly65, Ser66, Phe67, Gly68,
Val70, Lys85, Leu132, Val135, Asp181, Lys183, Gln185,
Asn186, and Asp200 in the active pocket docked with dehydroabietylamine
at the torsional degree of freedom 0.5 U
with Lamarckian genetic algorithm showed the inhibition
constant of 315.6 9 10-9. But the inhibition constant of
nitrofurazone and CHIR_98014 was 34.76 9 10-6 and
4.64 9 10-6 respectively. Docking of dehydroabietylamine
to GSK3-b protein involved in Wnt signaling pathway
supported the research on wound healing property. The
above said studies explore the role of dehydroabietylamine
in wound healing activity.