Optimisation of Culture Conditions for Porphyridium purpureum and Characterisation of Algal Biomass for Value Added Products.

Shalini, K. (2014) Optimisation of Culture Conditions for Porphyridium purpureum and Characterisation of Algal Biomass for Value Added Products. [Student Project Report] (Submitted)

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Abstract

Porphyridium spp. is a red micro alga and is gaining importance as a source of valuable products viz., phycobiliproteins (PB) especially red coloured phycoerythrin, sulphated exopolysaccharides, and polyunsaturated fatty acids with potential applications in the food and pharmaceutical industries. In the present study, the nutritional stress and CO2 supplementation were studied for enhancement of lipid content, PUFA especially EPA content and biomass concentration. A two phase cultivation protocol where the Porphyridium purpureum was first grown in growth enhancing Modified Medium for two weeks followed by two week incubation in highly saline Artificial Sea Water medium was found to enhance the lipid content to 18% with an EPA content of 19% and biomass concentration was 0.97g/L compared. The other major fatty acids observed were Palmitic, oleic and arachidonic acid. Supplementation with 5% CO2 was found to be growth enhancing with a significantly enhanced EPA content to 25%. Water, sodium phosphate buffer and acetic acid–sodium acetate buffer were used a extraction medium for phycoerythrin and purity of phycoerythrin was found to be more in water (0.9%) than acetic acid buffer (0.7%) and sodium phosphate buffer (0.79%). Phycoerythrin content was double in the culture grown on Modified medium as compared to the culture grown in Artificial Sea water medium. Protein content of crude phycoerythrin extract was 28.9%. Characterization of phycoerythrin was done by SDSPAGE. The antioxidant property of phycoerythrin was carried out and it was observed that the total antioxidant activity in water extract was more compared to acetic acid-sodium acetate buffer. Reducing power assay showed an increase in reducing activity with increase in concentration which indicates good reducing capacity.

Item Type:	Student Project Report
Uncontrolled Keywords:	Porphyridium, red micro alga, phycoerythrin, food industries, pharmaceutical industries
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 01 Algae 600 Technology > 08 Food technology
Divisions:	Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	26 Jun 2015 08:22
Last Modified:	26 Jun 2015 08:22
URI:	http://ir.cftri.res.in/id/eprint/11897

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