Fluorescence Based Turn-on Probe for the Determination of Caffeine Using Europium-Tetracycline as Energy Transfer Complex

Shwetha, N. and Honnur, Krishna and Praveena, Bhatt (2016) Fluorescence Based Turn-on Probe for the Determination of Caffeine Using Europium-Tetracycline as Energy Transfer Complex. Journal of Fluorescence (26). pp. 1115-1121.

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Abstract

The study describes a simple and sensitive fluorometric sensor based on the enhancement of fluorescence intensity of Europium ion (Eu3+) - tetracycline (TC) charge transfer complex on addition of caffeine. The Eu3+-TC ternary complex has a characteristic emission peak at 615 nm (λex = 375 nm), the intensity of which increases with increase in concentration of caffeine. The caffeine sensor assay was found to be linear in the range of 0.0515 mM to 51.5 mM. The limit of detection and quantification were found to be 0.0515 mM and 0.382 mM, respectively. A caffeine recovery of 90 to 110 % in biological samples (serum and urine) indicated minimal interference by commonly present excipients in the samples. Rosenthal plots to calculate the binding capacity of caffeine with the Eu3+- TC complex revealed an association constant (K) of 238 x 103 L/mol and binding number (N) of 1.9. Bland-Altman plot comparing the developed assay and HPLC showed good agreement between values obtained by both the methods. The proposed fluorescent chemical sensor is a rapid and convenient method to determine caffeine with excellent recovery and low detection limit. The probable reaction mechanism for the formation of the turn on fluorescent probe enhancer is discussed.

Item Type:	Article
Uncontrolled Keywords:	Eu3+- TC fluorescent probe . Caffeine . Turn-on assay . Binding number . Association constant . Analytical validation
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 18 Phytochemistry 600 Technology > 07 Beverage Technology > 04 Coffee
Divisions:	Fermentation Technology and Bioengineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	10 Aug 2016 12:29
Last Modified:	10 Aug 2016 12:29
URI:	http://ir.cftri.res.in/id/eprint/12247

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