Simon, Daris Pazhukunnel and Anila, N. and Mallikarjun Gouda, K. G. and Sarada, R. (2015) Vir gene inducers in Dunaliella salina; an insight in to the Agrobacterium-mediated genetic transformation of microalgae. Algal Research, 11. pp. 121-124.
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Abstract
Phenolic compounds such as vanillin, vanillic acid, gallic acid, coumarin, caffeic acid and acetosyringone, extracted
and identified from spent medium of Dunaliella salina has shown to facilitate the virulence (vir) gene induction
in Agrobacterium strain A348 (harboring pSM358, a virE::LacZ fusion vector). Among these phenolic
compounds, vanillin showed highest concentration of 38.12 ± 0.88 μg L−1 in spent medium. All these phenolic
compounds were evaluated independently for induction of vir gene expression using β-galactosidase activity.
Our results showed that except for gallic acid, therewas significant vir gene induction activity and these phenolic
compounds may be involved in facilitating efficient Agrobacterium-mediated transformation in D. salina.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Acetosyringone D. salina Vanillin Agrobacterium-mediated gene transfer Vir gene |
| Subjects: | 500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 01 Algae |
| Divisions: | Plant Cell Biotechnology |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 28 Nov 2016 07:31 |
| Last Modified: | 28 Nov 2016 07:31 |
| URI: | http://ir.cftri.res.in/id/eprint/12572 |
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