Production, statistical optimization and application of endoglucanase from Rhizopus stolonifer utilizing coffee husk

Navya, P. N. and Pushpa, S. Murthy (2013) Production, statistical optimization and application of endoglucanase from Rhizopus stolonifer utilizing coffee husk. Bioprocess and Biosystems Engineering, 36. pp. 1115-1123.

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Abstract

Coffee cherry husk (CH) is one of the major by-products obtained from coffee processing industry and accounts to 43 ± 5.9 % of cellulose. Screening of fungal organism for cellulase production was carried out and the potential organism was identified as Rhizopus stolonifer by internal transcribed spacer’s (ITS)—5.8S rDNA analysis. A systematic study with response surface methodology (RSM) based on CCRD was used to study the interactions among the variables such as pH (3–7), moisture (40–80 %) and progression duration (72–168 h) of the fermentation process to maximize the enzyme production. Under the optimized cultivation condition, R. stolonifer synthesized 22,109 U/gds. Model validations at optimum operating conditions showed excellent agreement between the experimental results and the predicted responses with a confidence level of 95 %. Endoglucanase thus produced was utilized for ethanol production by simultaneous saccharification and fermentation and maximum of 65.5 g/L of ethanol was obtained. This fungal cellulase has also reported to be efficient detergent additives and promising for commercial use. The present study demonstrates coffee husk as a significant bioprocess substrate. Statistical optimization with major parameters for cellulase production can be highly applicable for industrial scale. Furthermore, value addition to coffee husk with sustainable waste management leading to environment conservation can be achieved.

Item Type:	Article
Uncontrolled Keywords:	Coffee husk (CH) � Detergent compatibility � Endoglucanase � Response surface methodology (RSM) � Simultaneous saccharification and fermentation (ssf)
Subjects:	600 Technology > 07 Beverage Technology > 04 Coffee 600 Technology > 08 Food technology > 16 Nutritive value > 07 Waste utilization
Divisions:	Plantation Products Spices and Flavour Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	08 Dec 2016 07:28
Last Modified:	08 Dec 2016 07:28
URI:	http://ir.cftri.res.in/id/eprint/12580

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