Hexapeptide Fragment of Azurin Induces Apoptosis in Human Colon Carcinoma (Hct 116) Cell Line.

Nishita, K. P. and Shilpa, T. S. and Uma, V. Manjappara and Shylaja, M. Dharmesh and Manonmani, H. K. (2015) Hexapeptide Fragment of Azurin Induces Apoptosis in Human Colon Carcinoma (Hct 116) Cell Line. International Journal of Current Research and Academic Review, 3 (12). pp. 17-30.

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Abstract

Bioactive peptides, synthetic or natural have been studied as potential remedial agents against many human ailments, including cancer. However, the mode of action of these remedial peptides against cancer have not been clearly elucidated. In this paper, we discuss the effect of azurin derived hexapeptides on in vitro proliferation of human cancer cell lines (human colon carcinoma, HCT 116). The antiproliferative activities were evaluated using the MTT cell growth assay and apoptosis was confirmed by microscopic and FACS analysis. The hexapeptides studied exerted significant, dose and time related apoptotic effects. There was no elevation of p53 levels in treated cells. The penetration of peptide 4 into HCT116 was completely blocked by Nystatin and Filipin, inhibitors of caveolae-mediated endocytosis.

Item Type:	Article
Uncontrolled Keywords:	Hexapeptides Human colon carcinoma, HCT116, In vitro, Antiproliferative activity, Apoptosis
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 25 Peptide Chemistry
Divisions:	Dept. of Biochemistry Dept. of Lipid Science Food Protectants and Infestation Control
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	12 Jul 2017 05:46
Last Modified:	12 Jul 2017 05:46
URI:	http://ir.cftri.res.in/id/eprint/12811

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