Multiplex PCR assay for the detection of aflatoxigenic and non-aflatoxigenic Aspergilli.

Latha, R. and Manonmani, H. K. and Rati, E. R. (2008) Multiplex PCR assay for the detection of aflatoxigenic and non-aflatoxigenic Aspergilli. Research Journal of Microbiology, 3 (3). pp. 136-142.

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Abstract

Aflatoxins are potent secondary metabolites produced commonly by Aspergillus flavus, A. parasiticus and A. nomius. Primers were designed specifically for o-methyl transferase (omt) and aflatoxin regulatory gene (aflR) of aflatoxin biosynthetic pathway and also to detect the genus Aspergillus specific primers (18s rRNA genes) using NS. Experimental conditions were standardized for optimum multiplex PCR. DNA extracted from mycelia of toxigenic and non-toxigenic A. flavus, A parasiticus, other Aspergilli and from other genera of fungi were subjected to multiplex PCR using these primers. The omt and aflR primer pairs gave specific PCR amplification for aflatoxigenic A. flavus and A. parasiticus. They did not give DNA amplification for non-aflatoxigenic A. flavus, A. oryzae, A. glaucus, Fusarium, Penicillium and Rhizopus spp. Multiplex PCR assay for the detection of aflatoxigenic and non-aflatoxigenic Aspergilli. Available from:

Item Type:	Article
Uncontrolled Keywords:	aflatoxin, multiplex pcr, a.flavus
Subjects:	600 Technology > 08 Food technology > 09 Food Microbiology
Divisions:	Fermentation Technology and Bioengineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	24 Nov 2017 09:59
Last Modified:	24 Nov 2017 09:59
URI:	http://ir.cftri.res.in/id/eprint/13171

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