Engineered small intestinal system as an alternative to in-situ intestinal permeability model.

Intestinal permeability is the most important factor that determines the bioavailability. In-vitro techniques
like Caco-2 model and Ussing chamber are used for evaluating the intestinal permeability of the
bioactive compound. But the lack of mucosal layer in those models makes the intestinal absorption
prediction less accurate. We developed an engineered small intestinal system to evaluate intestinal
permeability of bioactive compound. Following this approach, we have evaluated the intestinal
permeability of fat soluble and water soluble bioactive compound and compared with in situ single-pass
intestinal perfusion technique.