Inhibition of metal catalyzed H2O2 and peroxyl-AAPH mediated protein, DNA and human erythrocytes lipid oxidation using millet phenolics.

Scientific data on the ability of the millet phenolic
profile in prevention of protein and human erythrocyte
peroxidation in terms of their cytoprotective properties
is scarce. Catechin, ferulic acid and traces of vanillic acid
and resveratrol were identified as bound polyphenols. It
was determined that all millet varieties bound phenolics
prevented DNA oxidation at a lower concentration of
50 lg. At a concentration of 25 lg kodo millet phenolics
retained 80% of proteins visualized in SDS-PAGE. Moreover
millet phenolics delayed time response for hemolysis
and showed an 88.2% inhibition of erythrocyte lipid peroxidation.
Though higher antioxidant property was estimated
in kodo millet their bioavailability may be affected
since much of the polyphenols occurred in bound form or
as condensed tannins. Processed kodo millet with increased
bioavailable phenolic content would thus be considered
effective compared to other millet varieties for its cytoprotective
properties.