Kannan, Rangiah and Palakodeti, Dasaradhi (2018) Quantification of Neurotransmitters from Intact and Regenerating Planarians Using UHPLC-MS/SRM Method. In: Planarian Regeneration: Methods and Protocols, Methods in Molecular Biology. Springer, pp. 555-570.
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Abstract
Freshwater planarian species S. mediterranea is an emerging stem cell model because of its capability of
regenerating large portions of missing body parts. It is one of the best model systems available to address
the basic biological mechanisms in the regeneration processes. Absolute quantification of metabolites from
planarians is imperative to understand their role in the regeneration processes. Here we describe a stable
isotope dilution ultrahigh performance liquid chromatography/mass spectrometry/selected reaction
monitoring (UHPLC-MS/SRM) assay for a sensitive and quantitative assessment of neurotransmitters
(NTs) in planaria. We used this method for the simultaneous quantification of 16 NTs from both intact
and regenerating planarians.
| Item Type: | Book Section |
|---|---|
| Uncontrolled Keywords: | Neurotransmitters, Quantification, Mass spectrometry, Planaria, Regeneration |
| Subjects: | 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 06 Chromatography 600 Technology > 08 Food technology > 28 Meat, Fish & Poultry > Fish |
| Divisions: | Food Safety Analytical Quality Control Lab |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 27 Aug 2018 10:30 |
| Last Modified: | 27 Aug 2018 10:30 |
| URI: | http://ir.cftri.res.in/id/eprint/13817 |
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