In vitro adhesion and anti-inflammatory properties of native Lactobacillus fermentum and Lactobacillus delbrueckii spp.

Aims: This study aimed at characterizing the adhesion and immunestimulatory
properties of native probiotic Lactobacillus fermentum (MCC 2759
and MCC 2760) and Lactobacillus delbrueckii MCC 2775.
Methods and Results: Adhesion of the strains was assessed in Caco-2 and HT-
29 cell lines. Expression of adhesion and immune markers were evaluated in
Caco-2 cells by real-time qPCR. The cultures displayed >80% of adhesion to
both cell lines and also induced the expression of mucin-binding protein
(mub) gene in the presence of mucin, bile and pancreatin. Adhesion was
mediated by carbohydrate and proteinaceous factors. The cultures stimulated
the expression of inflammatory cytokines in Caco-2 cells. However, proinflammatory
genes were down-regulated upon challenge with
lipopolysaccharide and IL-10 was up-regulated by the cultures. Cell wall extract
of L. fermentum MCC 2760 induced the expression of IL-6 by 5�47-fold,
whereas crude culture filtrate enhanced the expression of IL-10 by 14�87-fold
compared to LPS control.
Conclusions: The bacterial cultures exhibited strong adhesion and antiinflammatory
properties.
Significance and Impact of the Study: This is the first report to reveal the role
of adhesion markers of L. fermentum and L. delbrueckii by qPCR. The strainspecific
anti-inflammatory property of native cultures may be useful to alleviate
inflammatory conditions and develop a target-based probiotic.