Evaluation of anti-quorum sensing and antimutagenic activity of 2,3- unsaturated and 2,3-dideoxyglucosides of terpene phenols and alcohols.

The anti-quorum sensing property of terpene phenols/alcohols and their deoxyglucosides was evaluated qualitatively
against Pseudomonas aeruginosa and Chromobacterium violaceum by agar well diffusion method, and
their antimutagenic activity was tested by Ames test. The 2,3-unsaturated and 2,3-dideoxy 1-O-glucoside derivatives
demonstrated higher inhibitory zones compared to those for the parent molecules. MIC values indicated
that the derivatives of carvacrol (1.0–1.2 μmol/ml) and thymol (1.0–1.2 μmol/ml) were more effective than the
other compounds (1.2–2.0 μmol/ml). The anti-quorum sensing potential of derivatives was demonstrated by the
reduction in the production of pyocyanin (75–95%) and violacein (76–98%) at sub-MIC concentrations. The 2,3-
unsaturated 1-O-glucosides of carvacrol and thymol exhibited superior antimutagenic properties, and 2,3-unsaturated
1-O-glucoside of thymol showed 87.7% inhibition of MMS induced mutation in Salmonella typhimurium
TA 1538 at 1 μmol/plate concentration. The superior anti-quorum and antimutagenic activities exhibited by
these derivatives indicate their potential application as food preservatives.