Efficacy of Various Methods of Protein Extraction from Purslane Suitable for Proteome Analysis.

The purslane leaf is characterized by high content of protein, vitamins and minerals. However, its use
is limited due to the high fiber content and anti-nutritional substances. In this perspective, the objective of this research
was to optimize the protein extraction processes, aiming the use of purslane leaves as an alternative protein. The
important characteristics of the protocol include, 1) coagulation of proteins with mild acid precipitation, 2) extraction
of protein by three-phase partitioning, 3) extraction of protein by TCA/Acetone method. The protein concentrations
among three methods were 802±0.2 µg/g in mild acid precipitation, 625±0.3 µg/g in TCA Acetone and 81.6±0.2 µg/g in
TPP, respectively. The solubilization of proteins (method 1) showed a higher extraction yield. Based on the literature
survey, the comparison of protocol for protein extraction from purslane leaf was demonstrated first time in this work
and showed reproducible results.