Fatty acids influence the efficacy of lutein in the modulation of acrystallin chaperone function: Evidence from selenite induced cataract rat model.

Smitha, P. and Baskaran, V. (2020) Fatty acids influence the efficacy of lutein in the modulation of acrystallin chaperone function: Evidence from selenite induced cataract rat model. Biochemical and Biophysical Research Communications, 529. pp. 425-431. ISSN 0006-291X

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Abstract

Background: Loss of a-crystallin chaperone function results in the lens protein aggregation leading to cataract. In this study, we evaluated the efficacy of micellar lutein with different fatty acids in modulating a-crystallin chaperone function under selenite cataract conditions. Methods: Cataract was induced in rat pups by giving sodium selenite (25 mM/kg body weight) by IP. Lutein [(L), 1.3 mmol/kg body weight)] was given day before and five days after selenite injection as a micelle with 7.5 mM linoleic acid (LA), or 7.5 mM eicosapentaenoic acid (EPA)þdocosahexaenoic acid (DHA) or 7.5 mM oleic acid (OA). Lens a-crystallins was purified, and its chaperone function and integrity was assessed. Cholesterol, calcium, calpain-2, procaspase-3, and expression of a-A and b-B1 crystallin in the lens of cataract and micellar lutein administered rats were evaluated. Results: Cataract induction significantly (p < 0.05) decreased lens a-crystallin chaperone function. Cataract rats had increased cholesterol and calcium level, increased the expression of calpain-2, and a-A and b-B1 crystallin, and reduced the pro-caspase-3 level in the lens. However, micellar lutein administration significantly (p < 0.05) protected client proteins from aggregation via the modulation of calciumdependent calpain-2 protease activity. The chaperone function of lens a-crystallins in rats administered micellar lutein with EPA þ DHA was found to be highest when compared to OA and LA. Conclusions: Micellar lutein with unsaturated fatty acids beneficially modulates a-crystallin chaperone function. Among the fatty acids tested, micellar lutein with EPA þ DHA exhibited superior effects, thereby offering a promising strategy for cataract management.

Item Type:	Article
Uncontrolled Keywords:	Cataract Chaperone function Crystallins Fatty acids Lutein
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 14 Carotenoid Chemistry 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 17 Fatty Acid Chemistry
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	27 Nov 2020 04:44
Last Modified:	27 Nov 2020 04:44
URI:	http://ir.cftri.res.in/id/eprint/14653

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