In vitro anti-inflammatory property of a Quercetin-3-O-diglucoside-7-O-glucoside characterized from fresh leaves of Trigonella foenum-graecum L.

Kavya Bhatt, S. and Manjunatha Javagal, R. and Shashirekha, M. N. and Sachin, M. Eligar (2021) In vitro anti-inflammatory property of a Quercetin-3-O-diglucoside-7-O-glucoside characterized from fresh leaves of Trigonella foenum-graecum L. International Journal of Food Properties,, 24 (1). pp. 1438-1452.

PDF 10942912.2021.pdf - Published Version Restricted to Registered users only Download (1MB) | Request a copy

Abstract

Trigonella foenum-graecum L. (fenugreek) leaves are nutritionally rich green leafy vegetables consumed in South-East Asian and Middle-Eastern countries. Scientific data suggests properties including anti-inflammatory, antipyretic, anti-nociception, and hepatoprotective. However, the molecules responsible for these properties are yet to be identified. In the present study, we have isolated and characterized an anti-inflammatory nutraceutical molecule based on bioassay from the fresh leaves of fenugreek. The lipoxygenase inhibition assay revealed 79.9 ± 1.1% and 69.0 ± 2.9% inhibition in ethanolic and aqueous extracts, respectively. The ethanolic extract purified on a semi-prep HPLC column yielded a nutraceutical molecule quercetin glycoside (QG) having potent inhibition of lipoxygenase (75.3 ± 1.6%) at 10 mM and hyaluronidase (67.4 ± 4.0%) at 2 mM, respectively. The QG structure was elucidated by spectroscopic data, including FTIR, UHPLC-MS /MS, and 1D and 2D NMR as Quercetin-3-O-diglucoside-7-O-glucoside. QG also suppressed ROS, NO, and IL-6 production in LPS stimulated RAW264.7 cells. Radical scavenging assays of DPPH and ABTS revealed the potent antioxidant activity of QG with an EC50 of 245.5 and 68.8 μM, respectively. Our results demonstrate that newly characterized quercetin glycoside from fresh leaves of fenugreek possesses potential anti-inflammatory and antioxidant activities.

Item Type:	Article
Uncontrolled Keywords:	Fenugreek fresh leaves; Trigonella foenum-graecum L.; Anti-inflammatory; Lipoxygenase inhibition; Quercetin-3-O-diglucoside- 7-O-glucoside
Subjects:	600 Technology > 08 Food technology > 30 Spices/Condiments > 08 Fenugreek 600 Technology > 08 Food technology > 32 Antioxidants
Divisions:	Fruit and Vegetable Technology Plantation Products Spices and Flavour Technology Protein Chemistry and Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	29 Mar 2022 04:30
Last Modified:	29 Mar 2022 04:30
URI:	http://ir.cftri.res.in/id/eprint/15139

Actions (login required)

View Item