Delivery of Bioactive Molecule, b-Carotene - A Pro-Vitamin-A Carotenoid From Natural Source Using Microencapsulation Technique for its better Efficacy

Chanukya, B. S. and Pradeep, B. P. and Sagar, K. Bhat and Sindhuri, S. S. (2007) Delivery of Bioactive Molecule, b-Carotene - A Pro-Vitamin-A Carotenoid From Natural Source Using Microencapsulation Technique for its better Efficacy. [Student Project Report] (Submitted)

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type:	Student Project Report
Additional Information:	Carotenoid composition of spinach has been analyzed by HPLC and vitamin A activity (as retinol equivalent, RE) of Pro-vitamin A carotenoids was calculated. The level of b-carotene in spinach was 12.5mg/100g dry weight and its RE was 3976 (un-saponified) and 4113 (saponified). b-Carotene was purified using column chromatography and its purity was recorded as 96% against standard. The vitamin-A activity (RE) of purified b-carotene was 2083. Improving the bioavailability of b-carotene is vital to manage vitamin A deficiency. Initially, to find out the bioavailability of extracted b-carotene, vitamin-A deficiency rats were fed orally mixed micelles containing either with b-carotene or retinol. They were dissected after different time intervals, collected blood, and analyzed for the level of absorbed b-carotene. Results revealed that b-carotene and retinol level in plasma (43.2%, 44.5%) after two hours of feeding was higher in VAD animals than that of control group. the level of vitamin-A (retinal palmitate) was significantly higher in case of rats received retinol (49.96%) followed by β-carotene (44.34%) fed groups. In order to protect the b-carotene from oxidation and as well as to enhance its bioavailability, it was microencapsulated adopting spray-drying technique. The product was found to be free flowing and white in color. The structure of micro-encapsules was examined by phase contrast microscope. Its physicochemical properties showed slight variations at different temperatures. In addition to the above work, various biochemical analytical techniques namely cholesterol, phospholipid, lipid per-oxidation, and triglycerides were studied. From the results it is concluded that the product can be delivered successfully from natural sources using micro encapsulation by spray drying technique.
Uncontrolled Keywords:	carotenoids Vitamin A deficiency b-Carotene
Subjects:	600 Technology > 08 Food technology > 23 Vegetables 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 14 Carotenoid Chemistry
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	30 Nov 2007 10:49
Last Modified:	03 Jul 2015 12:19
URI:	http://ir.cftri.res.in/id/eprint/1521

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