Woodfordia fruticosa extract supplementation stimulates the growth of L. casei and L. rhamnosus with adapted intracellular and extracellular metabolite pool.

Shubha, J. R. and Pratima, Tripathi and Somashekar, B. S. and Kurrey, N. K. and Praveena, Bhatt (2021) Woodfordia fruticosa extract supplementation stimulates the growth of L. casei and L. rhamnosus with adapted intracellular and extracellular metabolite pool. Journal of Applied Microbiology. ISSN 1364-5072

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Abstract

Aim: To investigate the effect of Woodfordia fruticosa extract (WfE) on two probiotic bacteria Lacticaseibacillus casei and Lacticaseibacillus rhamnosus. Methods and Results: WfE supplementation at 0.5 mg ml-1 and 1 mg ml-1 stimulated probiotic growth (p < 0.05), enhanced adhesion to CaCO2 cells (p < 0.05) while inhibiting foodborne pathogens Escherichia coli and Staphylococcus aureus (p < 0.05). 1H-NMR based metabolomic studies indicated higher glucose:lactate and glucose:acetate in the extracellular matrix with significant variation (p < 0.05) in intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine and branched-chain amino acids and total cellular lipid composition. FAME analysis showed a chemical shift from saturated to unsaturated lipids with WfE supplementation. PCA plots indicated clear discrimination between test groups, highlighting variation in metabolite pool in response to WfE supplementation. Conclusion: Phytonutrient-rich WfE exhibited prebiotic-like attributes and probiotic bacteria showed altered metabolite pools as an adaptive mechanism. Significance and Impact of the study: This is the first report providing insights into the prebiotic�like activity of WfE on gut representative probiotics. The extended metabolomic studies shed light on the positive interaction between phytonutrients and beneficial bacteria that possibly help them to adapt to a phytonutrient-rich WfE environment. WfE with potential prebiotic attributes can be used in the development of novel synbiotic functional products targeting gut microbial modulation to improve health.

Item Type:	Article
Uncontrolled Keywords:	Woodfordia fruticosa, prebiotics, probiotics, phytonutrients, 1H NMR- metabolomics
Subjects:	600 Technology > 03 Agriculture > 04 Medicinal Plants 600 Technology > 08 Food technology > 09 Food Microbiology
Divisions:	Food Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	15 May 2023 11:26
Last Modified:	15 May 2023 11:26
URI:	http://ir.cftri.res.in/id/eprint/16347

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