Antimicrobial compound from microorganism of marine origin and elucidation of their characteristics.

Antimicrobial peptides (AMPs) are effective antimicrobial agents found in
plants, animals and microorganisms. The present work focuses on AMPs from marine�derived bacteria and their potential to control foodborne pathogens. We have screened
and isolated AMP producing bacteria (5 nos.) from the West coast region of South
India. The Box-Behnken design was applied to optimize the process variables for
maximum production of antibacterial compounds by the selected bacterial isolates.
After optimization, the two cultures (FTL6, FTL7) with a comparatively high fold
increase in AMP production were selected for further studies. Phylogenetic analysis
based on 16S rRNA gene sequences revealed that bacterial isolate FTL6 is closely
related to B. subtilis, and FTL7 is closely related to B. velezensis. Purification of AMP
from FTL6 and FTL7 was carried out and characterized. Through genome annotation,
the presence of genes responsible for the synthesis of AMP produced by FTL7 was
identified. Further, in silico studies supported the efficiency of these AMPs as
antibacterial agents in the food industry.