Oxidative alterations induced by D-aspartic acid in prepubertal rat testis in vitro: a mechanistic study.

Chandrashekar, K. N. and Dr., Muralidhara (2008) Oxidative alterations induced by D-aspartic acid in prepubertal rat testis in vitro: a mechanistic study. Theriogenology, 70 (1). pp. 97-104. ISSN 0093-691X

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Abstract

The objective of the present study was to investigate the oxidative induction response following in vitro treatment with D-aspartic acid (DA) in prepubertal rat testis (homogenates, explants, and cell suspensions). In all three preparations, DA enhanced (P<0.001) lipid peroxidation, manifest as increased reactive oxygen species (ROS) and malondialdehyde (MDA). Further, DA-induced oxidative induction was potentiated (P<0.001) in the presence of iron (5 microM) and 3-amino triazole and mercaptosuccinate (P<0.001), known inhibitors of the peroxide metabolizing enzymes, catalase and glutathione peroxidase, respectively. Testis homogenates exposed to L-arginine (LA) per se had reduced (P<0.001) endogenous levels of ROS and MDA; furthermore, pre-incubation with L-arginine markedly suppressed (P<0.001) DA-induced oxidative induction, suggesting an antagonistic action, perhaps due to LA-derived nitric oxide. In conclusion, DA caused significant oxidative induction in prepubertal rat testis, but this action was abrogated by L-arginine. The relevance of this phenomenon in vivo merits further study, as both of these molecules have specific physiological functions in the testis.

Item Type:	Article
Uncontrolled Keywords:	D-Aspartic acid (DA); Prepubertal testis; Oxidative response; L-Arginine; Steriodogenesis
Subjects:	600 Technology > 08 Food technology > 11 Food Biochemistry
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	17 Feb 2011 07:34
Last Modified:	09 May 2012 04:52
URI:	http://ir.cftri.res.in/id/eprint/1792

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