Prebiotic and Antiproliferation Effects of Arabinogalactan Isolated from Green Gram (Vigna radiata) and Its Hydrolysates.

Special Article: Legumes
Prebiotic and Antiproliferation Effects of Arabinogalactan
Isolated from Green Gram (Vigna radiata) and Its
Hydrolysates
Kiranmayi Ketha; Muralikrishna Gudipati*
Department of Biochemistry, CSIR- Central Food
Technological Research Institute, Mysuru, Karnataka,
India
*Corresponding author: G Muralikrishna
FAFST (I), FACCT(I) MNASc, Formerly Chief Scientist
(Scientist-G) & Formerly HOD, Professor AcSIR, Depart-
ment of Biochemistry, CSIR-Central Food Technological
Research Institute, Mysuru 570020, Karnataka, India.
Tel: +91-821-2516531 (R) Mobile (0) +91-9480382199
Email: krishnagm2002@yahoo.com
Received: May 30, 2024
Accepted: June 25, 2024
Published: July 02, 2024
Annals of Agricultural & Crop Sciences
Volume 9, Issue 4 (2024)
www.austinpublishinggroup.com
Gudipati M © All rights are reserved
Citation: Ketha K, Gudipati M. Prebiotic and Antiproliferation Effects of Arabinogalactan
Isolated from Green Gram (Vigna radiata) and Its Hydrolysates. Ann Agric Crop Sci. 2024;
9(4): 1160.
Annals of Agricultural & Crop Sciences
Open Access
Abstract
An Arabinogalactan (AGP) derived from green gram and its hy-
drolysates (AGO, Arabinogalacto oligosaccharides) were screened
for their prebiotic potential and antiproliferation effects on co-
lon cancer cells (in vitro). Three lactobacilli strains namely L. aci-
dophilus, L. delbrucki and L. fermentum showed relatively better
response towards AGP and AGO as compared with other tested
strains with respect to defined prebiotic characteristics. The ac-
tivities of α-L-arabinofuranosidase (39 to 89.5 mU/mL) and β-D-
galactopyranosidase (19 to 49.5 mU/mL) in the culture broth in-
dicated the breakdown of AGP and AGO into constituent sugars
and their subsequent utilization for the growth of bacteria. AGP
and AGO exhibited dose dependent cytotoxic and anti-proliferative
effects on (p <0.001) Caco-2 cells with IC50 values of 150 and 500
μg/mL, respectively as estimated by MTT assay. The present study
demonstrated the prebiotic properties of AGP and AGO as well as
their ability to provide natural defence against colon cancer.