Dietary supplementation with thymoquinone inhibits rotenone induced developmental toxicity in Drosophila melanogaster: Focus on ovary and larval development.

Pramod Kumar, P. and Saliya, S. Bawani and Usha Anandhi, Duraiswamy and Harish Prashanth, K. V. (2024) Dietary supplementation with thymoquinone inhibits rotenone induced developmental toxicity in Drosophila melanogaster: Focus on ovary and larval development. Food Bioscience, 62. p. 105233.

PDF 1-s2.0-S2212429224016638-main.pdf - Published Version Restricted to Registered users only Download (9MB) | Request a copy

Abstract

Pesticide exposure has been associated with various health conditions like asthma, Parkinson’s disease, Alzheimer’s disease, genetic disorders, epigenetic changes, oxidative stress, and hormonal disruption. Recent evidence suggests that infants are more vulnerable to pesticides (such as rotenone) than adults. Although the disease has already been clinically characterized, no treatment has yet been developed to improve pathology or prevent developmental toxicity. Therefore, the purpose of this study was to investigate whether thymoquinone (TQ) from Nigella sativa seeds could ameliorate rotenone (ROT)-induced developmental damage in both Drosophila melanogaster ovary and third instar larvae. Female adult wild-type flies and third instar larvae (72 ± 2 h after egg laying) were exposed to ROT and/or TQ through food for 48 and 24 h respectively. Results indicate that coexposure of TQ inhibits ROT-induced increased oxidative stress and alleviates abnormal antioxidant enzyme activities. Further, TQ alters developmental gene expression (Bacoid, Oscar, Nanos, and Gurken), impedes mitochondrial dysfunction, and reduces apoptosis in ROT induced ovary. In addition, TQ changes ROT induced abnormal crawling behaviour, cholinergic dysfunction, and dopamine depletion to normal levels in third instar larvae. Also, TQ reduces pupation and emergence time in ROT induced third instar larvae. The above findings demonstrate that ROT exposure during early developmental stages is inevitable, and TQ supplementation prevents developmental toxicity in the Drosophila ovary and third instar larvae. A diet enriched TQ provides a ray of hope in preventing ROT induced developmental toxicity.

Item Type:	Article
Uncontrolled Keywords:	Rotenone Thymoquinone Developmental toxicity Drosophila melanogaster Dopamine
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 26 Pesticide Chemistry 600 Technology > 01 Medical sciences > 17 Toxicology
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	08 Nov 2024 10:58
Last Modified:	08 Nov 2024 10:58
URI:	http://ir.cftri.res.in/id/eprint/18446

Actions (login required)

View Item