Traditionally used edible medicinal plants protect against rotenone induced toxicity in SH-SY5Y cells-a prospect for the development of herbal nutraceuticals

Aruna Chanu, Hijam and Yaiphabi Chanu, Tongbram and Pooja Devi, Nongthombam and Heikrujam Nilkanta, Meitei and Arunkumar Singh, Koijam and Yallapa, Rajashekar and Reena, Haobam (2024) Traditionally used edible medicinal plants protect against rotenone induced toxicity in SH-SY5Y cells-a prospect for the development of herbal nutraceuticals. Neurochemistry International, 180. pp. 1-13.

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Abstract

Plants are good sources of pharmacologically active compounds. The present study aimed to examine the neu­ roprotective potentials of the methanol extracts of Salix tetrasperma Roxb. leaf (STME) and Plantago asiatica L. (PAME), two edibles medicinal plants of Manipur, India against neurotoxicity induced by rotenone in SH-SY5Y cells. Free radical quenching activities were evaluated by ABTS and DPPH assays. The cytotoxicity of rotenone and the neuronal survival were assessed by MTT assay and MAP2 expression analysis. DCF-DA, Rhodamine 123 (Rh-123), and DAPI measured the intracellular reactive oxygen species (ROS) levels, mitochondrial membrane potential (MMP), and apoptotic nuclei, respectively. Superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) activities were also assessed. LC-QTOF-MS analysis was performed for the identification of the compounds present in STME and PAME. The study showed that both the plant extracts (STME and PAME) showed antioxidant and neuroprotective capabilities in rotenone-induced neurotoxicity by preventing oxidative stress through the reduction of intracellular ROS levels and reversing the activities of GPx, SOD, and CAT caused by rotenone. Further, both plants prevented apoptotic cell death by normalizing the steady state of MMP and protecting nuclear DNA condensation. LC-QTOF-MS analysis shows the presence of known neuroprotective compounds like uridine and gabapentin in STME and PAME respectively. The two plants might be an important source of natural antioxidants and nutraceuticals with neuroprotective abilities. This could be investigated further to formulate herbal nutraceuticals for the treatment of neurodegenerative disease like Parkinson’s disease.

Item Type:	Article
Uncontrolled Keywords:	Neuroprotection, Antioxidant, Salix tetrasperma, Plantago asiatica, Herbal nutraceuticals, SH-SY5Y cells, Rotenone
Subjects:	600 Technology > 01 Medical sciences > 17 Toxicology 600 Technology > 03 Agriculture > 04 Medicinal Plants
Divisions:	Food Protectants and Infestation Control
Depositing User:	Somashekar K S
Date Deposited:	05 Mar 2025 10:31
Last Modified:	05 Mar 2025 10:31
URI:	http://ir.cftri.res.in/id/eprint/19180

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