Carotenoid production by Formosa sp. KMW, a marine bacteria of Flavobacteriaceae family: Influence of culture conditions and nutrient composition

Exploitation of microbial sources for production of carotenoids is important as an alternative to synthetic
carotenoids. The carotenoid production by microorganism is dependent on culture conditions and the
nutrients. In this study, the carotenoid production by the marine bacteria Formosa sp. KMW belonging to
Flavobacteriaceae family was evaluated under different growth conditions and nutrient composition. The
yield of the total carotenoid produced increased with incubation time and the zeaxanthin content in-
creased to 51.47 1.5% of total carotenoids after 6 days of incubation. The total carotenoid production was
highest (0.9770.03 mg/L) at pH 7.0. Carotenoid extract yield and carotenoid content were higher when
the culture was grown under shaking condition under light. Significantly higher carotenoid production
was observed in the absence of sugars (0.69 70.14 mg/L). The yield of carotenoid significantly (po 0.05)
differed when culture was grown in the presence of different amino acids. TCA cycle intermediates did
not benefit the carotenoid production. Higher carotenoid yield was obtained when vortexing was used as
a method of extraction. The study indicated that the carotenoid production in Formosa sp. is greatly
affected by the growth condition and the nutrient composition of the growth media and the organism
can be exploited for the production of zeaxanthin.