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UHPLC-MS/MS based comprehensive phenolic profiling, antimicrobial and antioxidant activities of Indian Rhodomyrtus tomentosa fruits
Divya Priya, A. and Asha, Martin (2025) UHPLC-MS/MS based comprehensive phenolic profiling, antimicrobial and antioxidant activities of Indian Rhodomyrtus tomentosa fruits. Journal of Scientific Reports, 15. pp. 1-13.
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Abstract
Rhodomyrtus tomentosa fruits, endemic to the Western Ghats were analyzed for its free, bound and esterified phenolics by Ultra High Performance Liquid Chromatography–Mass Spectrometry. Overall, twenty-nine phenolic compounds were identified, amongst them 18 were detected in this fruit for the first time. Gallic acid (80.44 ± 8.74 mg/100 g) and ellagic acid (107.47 ± 7.28 mg/100 g) were the most prominent ones found in the bound phenolic fraction and gallic acid (103.76 ± 6.34 mg/100 g) in the esterified phenolic fraction of the fruit, respectively. Total Phenolic content was found to be highest in bound phenolics (7.09 ± 0.17 mg Gallic acid equivalent/g). The antioxidant and antimicrobial activities of the three extracts namely free, bound and esterified phenolic fruit fractions have been analyzed. Bound phenolics exhibited the highest antioxidant potential (DPPH-15.63 ± 0.86; ABTS-34.73 ± 0.07; FRAP-17.89 ± 0.27 mg/g Ascorbic acid equivalent). The bound phenolics showed good antimicrobial activity against Bacillus cereus, Staphylococcus aureus and Escherichia coli with a MIC of 0.156, 0.625 and 1.25 mg/mL respectively. The exploration of phenolic compounds in Indian variety of Rhodomyrtus tomentosa fruits may provide useful insights on its utilization as a functional food ingredient.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Indian Rhodomyrtus tomentosa, UHPLC-MS/MS, Antioxidant activity, Antimicrobial activity |
| Subjects: | 600 Technology > 08 Food technology > 24 Fruits 600 Technology > 08 Food technology > 32 Antioxidants |
| Divisions: | Dept. of Biochemistry |
| Depositing User: | Somashekar K S |
| Date Deposited: | 25 Jun 2025 06:41 |
| Last Modified: | 25 Jun 2025 06:41 |
| URI: | http://ir.cftri.res.in/id/eprint/19619 |
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