Enhanced Analysis of Curcuminoids in Turmeric via Selective Homodecoupled 1D 1 H NMR

Naresh, K. S. and Anisha, Biswas and Sachin Chaudhari, R. (2025) Enhanced Analysis of Curcuminoids in Turmeric via Selective Homodecoupled 1D 1 H NMR. Journal of Magnetic Resonance in Chemistry, 63. pp. 660-669.

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Abstract

Curcuminoids, including curcumin, demethoxycurcumin, and bisdemethoxycurcumin, are vital for quality control in food, nu- traceuticals, and pharmaceuticals. Conventional 1D 1 H NMR can face challenges in spectral interpretation when dealing with overlapping signals and complex coupling patterns, especially in structurally similar compounds like curcuminoids. This study explores the use of selective homodecoupled 1D 1 H NMR spectroscopy as a complementary technique to enhance spectral res- olution and facilitate peak assignment in curcuminoid analysis. By collapsing multiplet structures such as doublets observed in the 6.6- to 6.8-ppm region for vinylic protons into singlets, this method offers improved spectral clarity. Although absolute quantification still requires deconvolution, the approach aids in more straightforward relative integration and identification of components within curcuminoid mixtures from turmeric samples. The results demonstrate improved interpretability compared with conventional 1 H NMR under similar conditions. Comparative analysis with HPLC showed excellent agreement, with stand- ard deviations under 2% for most samples. The selective homodecoupled 1D 1 H NMR method proved robust and reliable, offering an effective tool for profiling curcuminoids and potential application to other natural product mixtures.

Item Type:	Article
Uncontrolled Keywords:	curcuminoids, HPLC, NMR spectroscopy, turmeric
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 18 Phytochemistry 600 Technology > 08 Food technology > 30 Spices/Condiments > 07 Turmeric
Divisions:	Plantation Products Spices and Flavour Technology
Depositing User:	Somashekar K S
Date Deposited:	07 Oct 2025 06:03
Last Modified:	07 Oct 2025 06:03
URI:	http://ir.cftri.res.in/id/eprint/19882

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