Phycobiliproteins of Marine Red Alga, Kappaphycus Alvarezii Possess Potent Antioxidant and Metal Chelating Properties, and Inhibit Hepatocellular Carcinoma (HepG2) Cell Growth

Gayathridevi, Mahendran and Nimish Mol, Stephen and Mangaiyarkarasi, Manivel and Tehreem, Maradagi and Muralidharan, Nagarajan and Jayakumar, Natarajan and Ganesan, Ponesakki (2025) Phycobiliproteins of Marine Red Alga, Kappaphycus Alvarezii Possess Potent Antioxidant and Metal Chelating Properties, and Inhibit Hepatocellular Carcinoma (HepG2) Cell Growth. Applied Biochemistry and Biotechnology. pp. 1-22.

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Abstract

Kappaphycus alvarezii is one of the economically important red alga, harvested primarily for the production of hydrocolloids. This study aimed to identify an effective extraction method for the isolation of phycobiliproteins (PBPs) from K. alvarezii and evaluate its biofunctional properties. Organic solvents, inorganic acids, organic acids, phosphate buf- fer saline (PBS), distilled water, and freeze-thawing were used to identify an effective extraction method. The identification of PBPs was carried out by UV-Vis spectrophotom- etry, FTIR spectroscopy, and SDS-PAGE analysis. The antioxidant potentials of PBPs was examined by DPPH and ABTS radical scavenging assays. The metal chelating efficiency was determined using divalent, monovalent, and trivalent metal ions. The WST-1 assay and DAPI staining were performed to examine HepG2 cell viability and apoptosis, re- spectively. Among the extraction methods, higher content of phycoeryhrin was recorded in phosphate buffer saline. The results showed that extraction using PBS exhibits highest content of phycoerythrin. The PBPs fraction contains phycoerythrin as a major pigment molecule. The isolated PBPs exhibited increased DPPH and ABTS radical scavenging activity in a concentration-dependent manner. It has possible quenching ability of Co2+ and Cu2+. The isolated PBPs inhibited HepG2 cell growth in a dose-dependent manner and induced nuclear fragmentation. The data emphasize that K. alvarezii could be utilized for the extraction of phycoerythrin which has antioxidant, metal chelation, and anticancer properties.

Item Type:	Article
Uncontrolled Keywords:	Marine red algae, Kappaphycus alvarezii, Nutritional composition, Extraction methods, Phycobiliproteins, Bio-functionality
Subjects:	600 Technology > 08 Food technology > 16 Nutritive value > 10 Antioxidant Property 600 Technology > 08 Food technology > 29 Microbiological food > 01 Algae
Divisions:	Dept. of Biochemistry Meat Fish and Poultry Technology Molecular Nutrition
Depositing User:	Somashekar K S
Date Deposited:	21 Nov 2025 06:50
Last Modified:	21 Nov 2025 06:50
URI:	http://ir.cftri.res.in/id/eprint/20102

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