Characterization of free oligosaccharides from garden cress seed aqueous exudate using PGC LC-MS/MS and NMR spectroscopy

Lijina, P. and Manjunatha, J. R. and Gnanesh Kumar, B. S. (2023) Characterization of free oligosaccharides from garden cress seed aqueous exudate using PGC LC-MS/MS and NMR spectroscopy. Carbohydrate Research, 532. pp. 1-7.

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Abstract

Garden cress seeds produces mucilage that has found various food applications, however, there is little infor- mation on the free oligosaccharides (FOS) contents in these seeds. Herein, we explored the presence of FOS in cress seed aqueous exudate. PGC-LC MS/MS analysis indicated the presence of mainly hexose containing oli- gosaccharides such as raffinose, stachyose and verbascose belonging to raffinose family of oligosaccharides (RFOs). In addition, minor fraction of planteose, isomeric tri- and tetrasaccharides were also observed. Further, the structural confirmation of the abundant tri- and tetrasaccharide were obtained through 1D and 2D NMR analysis. Thus, the RFOs presence in cress seeds would enhance its bio-functionalities.

Item Type:	Article
Uncontrolled Keywords:	Cress seeds, Free oligosaccharides, Isomers, Mass spectrometry, NMR, RFOs
Subjects:	600 Technology > 03 Agriculture > 04 Medicinal Plants 600 Technology > 08 Food technology > 01 Analysis
Divisions:	Central Instrumentation Facility Services Dept. of Biochemistry
Depositing User:	Somashekar K S
Date Deposited:	19 Dec 2025 05:15
Last Modified:	19 Dec 2025 05:15
URI:	http://ir.cftri.res.in/id/eprint/20178

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