Characterization of different parts of litchi fruit using UHPLC‑QExactive Orbitrap

Litchi fruit is consumed across the globe for
its high nutritional value and taste. The qualitative profil-
ing of litchi fruit has been carried out by using ultra-high-
performance liquid chromatography with QExactive high-
resolution accurate mass spectrometry. Acidified water:
methanol: acetonitrile (1:1:1) extracts from individual parts
(skin, pulp, and seed) of matured litchi, were subjected to
LC–MS analysis with electrospray ionization in full MS-
ddMS 2 mode as a non-target approach. The data was pro-
cessed through compound discoverer software by the use of
mzCloud and ChemSpider databases, for compound iden-
tification. We identified 77 compounds with protonated or
deprotonated forms based on the polarity and their charac-
teristic fragments are within ± 4 ppm mass error and reten-
tion time ± 0.1 min for parent and fragments. Hypoglycin B
is the first time reported in litchi fruit along with hypoglycin
A. Further, we verified the distribution of the identified com-
ponents and differentiation of three different parts of litchi
through principal component analysis.