Estimation of intracellular lipids by the measurement of absorbance of yeast cells stained with Sudan Black B

Thakur, M. S. and Prapulla, S. G. and Karanth, N. G. (1989) Estimation of intracellular lipids by the measurement of absorbance of yeast cells stained with Sudan Black B. Enzyme and Microbial Technology, 11 (4). pp. 252-254. ISSN 0141-0229

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Abstract

A rapid and sensitive method has been developed for the estimation of intracellular lipids accumulated in oleaginous yeast cells [Rhodotorula gracilis CFR 1 used for present studies]. The cells are stained with Sudan Black B and the absorbance is measured at 580 nm using unstained cells as control. A linear relationship is observed between absorbance and lipid concn. in fermentation broth. The values predicted using this relationship were compared with those obtained using the conventional method of solvent extraction followed by gravimetric estimation and a close agreement has been obtained.

Item Type:	Article
Uncontrolled Keywords:	LIPIDS-; yeasts, lipids intracellular estimation in; SPECTROSCOPY-; PRODUCT-TECHNOLOGY; YEASTS-; Rhodotorula gracilis, lipids intracellular estimation in; lipids intracellular estimation in yeasts
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 19 Yeast 600 Technology > 05 Chemical engineering > 01 Biotechnology and Bioengineering
Divisions:	Fermentation Technology and Bioengineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	05 Jul 2008 10:49
Last Modified:	10 Jan 2019 09:21
URI:	http://ir.cftri.res.in/id/eprint/2038

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