Enhanced biosynthesis of polyhydroxyalkanoates in a mutant strain of Rhizobium meliloti.

Lakshman, Kshama and Shamala, Tumkur Ramachandriah (2003) Enhanced biosynthesis of polyhydroxyalkanoates in a mutant strain of Rhizobium meliloti. Biotechnology Letters, 25 (2). pp. 115-119. ISSN 0141-5492

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Abstract

Strains of Rhizobium spp. isolated from leguminous plants and standard strains accumulated 27% to 57% polyhydroxyalkanoate (PHA) of their cell biomass. Among these cultures, one strain of Rhizobium meliloti synthesized 10-30% more PHA than others and contained 3% hydroxyvalerate (HV) when grown on sucrose as carbon substrate. The occurrence of hydroxybutyrate (HB) and HV was confirmed by GC and 1H NMR analysis. Treatment of the culture with 4'-N-piperidinobutyl-2-chlorophenoxazine resulted in a mutant which synthesized up to 69%, PHA of the cell biomass with an improved yield of 11 to 47% under different carbon and nitrogen ratios, compared to the parent strain.

Item Type:	Article
Uncontrolled Keywords:	Poly(hydroxybutyrate-co-hydroxyvalerate)-P(HB-co- HV); Rhizobium spp.; Polysaccharides; Mutation
Subjects:	600 Technology > 08 Food technology > 09 Food Microbiology
Divisions:	Food Microbiology
Depositing User:	Users 197 not found.
Date Deposited:	22 Jun 2011 10:57
Last Modified:	28 Dec 2011 09:37
URI:	http://ir.cftri.res.in/id/eprint/2060

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